Transformation and reversion: Pitfalls imposed by heterokaryosis

Heterokaryosis in Neurospora sitophila.

ETEROKARYOSIS has been extensively used as a major tool in formal genetic analysis of both Mendelian and non-Mendelian genes and in establishing gene-enzyme relationships. So far it is the only way to perform genetic anslysis of somatic cells in tissue culture (EPHRUSSI 1965; MINTZ and BAKER 1967). These aspects of heterokaryosis have been reviewed elsewhere ( JINKS 1965; FINCHAM 1966; DAVIS 19...

compactifications and representations of transformation semigroups

this thesis deals essentially (but not from all aspects) with the extension of the notion of semigroup compactification and the construction of a general theory of semitopological nonaffine (affine) transformation semigroup compactifications. it determines those compactification which are universal with respect to some algebric or topological properties. as an application of the theory, it is i...

Genetic Interaction among Streptomycetes: Heterokaryosis and Synkaryosis.

Heterokaryosis and alkaloid production in Claviceps purpurea.

Strain 275 FI of Claviceps purpurea, which produces large amounts of peptide alkaloids in submerged culture, is a heterokaryon; after several generations on agar media, it segregates the single components. These components (by us labeled V, C, and W) are stable and produce almost no alkaloids under described conditions of submerged culture. The mycelium of strain 275 FI consists of hyphae with ...

Maintaining heterokaryosis in pseudo-homothallic fungi

Among all the strategies displayed by fungi to reproduce and propagate, some species have adopted a peculiar behavior called pseudo-homothallism. Pseudo-homothallic fungi are true heterothallics, i.e., they need 2 genetically-compatible partners to mate, but they produce self-fertile mycelium in which the 2 different nuclei carrying the compatible mating types are present. This lifestyle not on...